Portable Biotech Skid

This photograph is of a Single-Tank By-Pass Portable CIP Skid equipped with a CIPS Valve Manifold which provides the means of supplying flush, wash and rinse solutions to the legs of small bioreactors, as well as to the spray device.  The PLC based control system includes software to perform Device Sequencing as a support routine run in parallel with the normal CIP program.  The spray device receives CIPS flow during the full CIP program, in accordance with normal practice for rinsing and draining vessels.  The five (5) additional CIPS valves are connected to the various legs of the bioreactor with small diameter Flexline tubing.  Connections include:

  • The Exhaust Line.
  • Two Long Dip Tubes cleaned in parallel.
  • Two Additive Lines cleaned in parallel.
  • Media, Inoculum and Sample Valve inlets.
  • A low-level sparger.
  • These legs are all equipped with manual valves which are fully opened before starting the CIP program.

BioPH04DMiniwCIPSMan

All CIPS flow is to the bioreactor and the single small puddle in the vessel drains through a larger diameter Flexline to the CIPR Sensor Manifold.  The Drain Valve on the CIP Skid is connected via Flexline to the facility biowaste system.

BioPH04FMiniwFloSeqClronW

A Typical Valve (device) Sequencing program would be as follows: 

1)  Spray the vessel full time.

2)  During the first 30 seconds of each minute, open one Leg CIPS valve at a time, allowing the CIPS flow to divide between the spray and the leg.

3)  After each pass through the legs in parallel with the spray, supply the spray only for the next 30 seconds of each minute.

4)  Repeat this procedure from the beginning to the end of the program, for all flushes, recirculation washes and rinses.

The device sequencing program can be expanded to control other components such as agitators or pumps in the circuit.

Discuss valve sequencing protocol here.